École Normale Supérieure
Seminar co-hosted by the departments of Chemistry and Biology
Friday June 26th, 10:30 a.m., library of IBPC, 13 rue Pierre et Marie Curie, Paris.
Development of Fluorescent Tools for Live Cell Imaging
Amy Palmer, Professor at University of Colorado Boulder
Our research lies at the interface of chemistry and biology, where the application of chemical and physical principles provides a unique opportunity to better understand the fundamental biochemistry of living cells. Cells are complex entities that must integrate internal and external signals in order to coordinate diverse functions. Living cells are also dynamic, and this dynamism is key to understanding the mechanisms between cause and effect for biological processes. Our lab develops new technologies to interrogate signaling cascades in cells to understand how the actions of specific proteins, molecules, and ions contribute techniques with protein design and engineering to develop novel fluorescent probes, and use long-term time-lapse fluorescence microscopy to elucidate the mechanisms of cellular to cellular function. We combine in vitro spectroscopic and biophysical signaling pathways. Longitudinal single-cell analyses also allow us to explore the origins and consequences of heterogeneity in biological systems. We are specifically interested in how cells regulate metal ions, how pathogens alter cell biology, and how to engineer improved photophysical properties in fluorescent proteins. In this talk I will discuss our lab’s efforts to develop fluorescent sensors based on fluorescence resonance energy transfer, the application of microfluidics technology to evolve new and improved fluorescent proteins, and recent progress in long-term time lapse microscopy of infected cells to dissect mechanisms of virulence.
For further details, please contact Arnaud Gautier (arnaud.gautier [@] ens.fr) or Alice Lebreton (alice.lebreton [@] ens.fr)